M.Sc. Lars Thiede

Function

doctoral candidate in the group of Charlotte Uetrecht

At UzL since

2024

Other affiliations

CSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY & Leibniz Institute of Virology (LIV) & University of Lübeck, Notkestraße 85, 22607 Hamburg, Germany.

Funding

ARIADNE VIBE
Horizon 2020 Research and Innovation program under grant agreement No. 964553

ORCID ID

0000-0002-2149-8368

CSSB Centre for Structural Systems Biology
c/o Deutsches Elektronen-Synchrotron DESY
Notkestraße 85, Building 15
22607 Hamburg
Gebäude CSSB, Raum O2.139

Email:	lars.thiede(at)uni-luebeck.de
Phone:	+49 040 8998 6372

Research and work

Research focus

assembly, stability, and structure of human norovirus capsids (VLPs)
biophysical principles underlying capsid formation and heterogeneity
structural variants and modified particles for potential biotechnological applications
mass distributions, particle heterogeneity, and assembly mechanisms in highly complex capsid samples

Methods & approaches

native mass spectrometry (MS) for analyzing assembly and stability
orbitrap-based charge-detection mass spectrometry (CDMS) for direct mass determination of heterogeneous megadalton particles
complementary structural methods such as single-particle cryo-electron microscopy and atomic force microscopy (AFM)
integration of multimodal biophysical data for detailed structural and dynamic analyses of viral capsid

Expertise

native MS for very high-mass complexes
orbitrap-based CDMS
Norovirus capsid structure & assembly
ion mobility mass spectrometry

Publications

Research portal of the University of Lübeck

CV

seit 2024	doctoral candidate	University of Lübeck, Institute for Chemistry and Metabolomics, Germany
2021-2024	doctoral candidate	Leibniz Institute of Virology, Hamburg & University of Siegen, Germany
2016-2019	master student (Biochemistry)	Martin Luther University Halle-Wittenberg, Germany
2013-2016	bachelor student (Biochemistry)	Martin Luther University Halle-Wittenberg, Germany