AVANCE III HD & AVANCE IVDr Platform
Manufacturer
Bruker Daltonics
Location
Institute of Chemistry and Metabolomics, Lübeck, Germany
Description
- 600 MHz, Ascend™ 600
- 5 mm TXI (1H/13C/15N)
- SampleJet automated sample changer
Applications
- standardized, high-throughput 1H-NMR metabolomics and glycoproteomics of human serum, plasma, urine, and other biofluids within clinical and longitudinal cohort studies, with minimal and automated sample preparation
- automated quantification of numerous small-molecule metabolites and comprehensive lipoprotein profiles using Bruker B.I.Quant-PS and B.I.LISA modules
- NMR-based glycoprotein characterization using the institute’s patented Glycoplatform method for the identification of biomarkers in inflammatory and oncological diseases
- method development for detecting diverse molecular species in various biological sample types to enable the discovery of novel biomarkers
AVANCE III HD & CryoPlatform
Manufacturer
Bruker Daltonics
Location
Institute of Chemistry and Metabolomics, Lübeck, Germany
Description
- 600 MHz, Ascend™ 600
- 1.7 mm TCI MicroCryoProbe (1H/13C/15N)
- 5 mm TCI CryoProbe (1H/13C/15N)
- SampleJet automated sample changer
Applications
- high-resolution structural analysis of proteins, glycoproteins, and glycans in solution (1D, 2D, and 3D NMR; e.g., HSQC, HMQC, NOESY, TOCSY, methyl-TROSY-HMBC) using both unlabeled and 13C/15N-labeled samples
- structural and dynamic characterization of protein–ligand and protein–glycan interactions, including studies on viral capsid proteins (e.g., noroviruses), using chemical shift analysis, STD-NMR, and related methods
- NMR-based metabolomics and metabolic-flux analysis in cell lysates and extracts (tracking of stable-isotope-labeled metabolites to resolve metabolic pathways)
- method development for highly sensitive experiments using very small sample volumes, enabling metabolomics across diverse biological sample types
AVANCE III HD FoodScreener
Manufacturer
Bruker Daltonics
Location
Institute of Chemistry and Metabolomics, Lübeck, Germany
Description
- 400 MHz, Ascend™ 400
- 5 mm BBI (1H/31P/15N)
- SampleXpress automated sample changer
Applications
- authenticity and quality assessment of food products (e.g., fruit juices, honey, spices) with minimal sample preparation
- detection of adulteration and origin mislabeling (e.g., added sugars, incorrect botanical or geographical origin, dilution, substitution of raw materials)
- training and teaching in NMR spectroscopy
- structural elucidation of small organic molecules and reaction monitoring in synthesis courses and laboratory practicals
Fourier 80 Benchtop
Manufacturer
Bruker Daltonics
Location
Institute of Chemistry and Metabolomics, Lübeck, Germany
Description
- 80 MHz
- Fourier 80 PAL automated sample changer
Applications
- method development to transfer established high-field metabolomics workflows to a benchtop system with simple maintenance and no reliance on cryogens, enabling future clinical applications
maXis II™ LC-QTOF
Manufacturer
Bruker Daltonics
Location
Institute of Chemistry and Metabolomics, Lübeck, Germany
Beschreibung
- ultra-high-resolution QTOF with high mass accuracy (accurate mass)
- quadrupole selection and TOF detection
- coupled to LC for analysis of complex samples
- MS/MS fragmentation via collision cell (CID))
Anwendungen
- mass spectrometry for analyses complementary to NMR spectroscopy, e.g., untargeted/targeted LC-MS-based metabolomics as well as peptide and protein analysis
8890 GC System & 5977C GC/MSD
Manufacturer
Agilent
Location
Institute of Chemistry and Metabolomics, Lübeck, Germany
Beschreibung
- GC–MS system (8890 gas chromatograph coupled to a 5977C single-quadrupole MSD)
- electron ionization (EI) for reproducible fragmentation and library matching
- full-scan and SIM modes for both screening and targeted analysis
Anwendungen
- analysis of volatile and semi-volatile compounds in complex matrices (e.g., clinical samples)
- metabolomics of low-abundance metabolites (complementary to NMR-based metabolomics)
