Q Exactive™ UHMR Hybrid Quadrupole Orbitrap™ & DMT
Manufacturer
Thermo Fisher Scientific
Location
CSSB Centre for Structural Systems Biology, Hamburg, Germany
Description
- enhanced with a Direct Mass Technology (DMT) module for single-ion detection
- designed for very high masses up into the megadalton range
- high resolution enabled by Orbitrap™ technology
- collision cell supporting argon or xenon
Applications
- native and top-down mass spectrometry
- structural characterization of large proteins and proteoforms, including viral particles
- charge-detection mass spectrometry (CDMS)
Q Exactive™ UHMR Hybrid Quadrupole Orbitrap™ & Omnitrap
Manufacturer
Thermo Fisher Scientific & Fasmatech
Location
Leibniz Institute of Virology, Hamburg, Germany
Description
- equipped with an Omnitrap ion trap enabling advanced fragmentation methods (CID, ECD/EID, photodissociation)
- a uniquely configured UHMR–Omnitrap system with no equivalent worldwide
- designed for very high masses up into the megadalton range
- high resolution enabled by Orbitrap™ technology
- collision cell supporting argon or xenon
Applications
- native and top-down mass spectrometry
- multistage fragmentation (MSn)
- structural characterization of large proteins and proteoforms, including viral particles
Q-Tof 2 hybrid quadrupole time-of-flight
Manufacturer
Waters/Micromass
MS Vision (Modifikationen)
Location
CSSB Centre for Structural Systems Biology, Hamburg, Germany
Description
- designed for very high masses, extending into the megadalton range
- broad mass range with high transmission for large complexes
- quadrupole selection combined with TOF detection
- collision cell operable with argon or xenon
Applications
- native and top-down mass spectrometry
- structural analysis of large proteins and proteoforms, including viral particles
- complements Orbitrap™-based systems for high-mass measurements
Q-Tof Ultima
Manufacturer
Waters/Micromass
MS Vision (Modifikationen)
Location
Hamburg Advanced Research Centre for Bioorganic Chemistry (HARBOR), Hamburg, Germany
Description
- custom-modified to enable measurements in the high-mass (megadalton) range
- adapted for coupling mass spectrometry to various X-ray sources (PETRA III, FLASH I/II, European XFEL)
- quadrupole selection combined with TOF detection
Applications
- native and top-down mass spectrometry
- MS-based sample injection for single-particle imaging (SPI)
- X-ray spectroscopy
MS SPIDOC
Manufacturer
Custom-built in collaboration with Fasmatech, MS Vision, the University of Greifswald, Germany, and the University of Manchester, UK
Location
Hamburg Advanced Research Centre for Bioorganic Chemistry (HARBOR), Hamburg, Germany
Description
- mass spectrometry–based sample injection for scattering experiments – suitable for individual proteins (kilodalton range) up to viral capsids (megadalton range)
- adapted for coupling MS with various X-ray sources (PETRA III, FLASH I/II, European XFEL)
- quadrupole selection, ion mobility, and TOF detection
Applications
- gas-phase scattering experiments with a focus on single-particle imaging (SPI), small-angle X-ray scattering (SAXS), and wide-angle X-ray scattering (WAXS)
timsTOF Pro
Manufacturer
Bruker Daltonics
Location
CSSB Centre for Structural Systems Biology, Hamburg, Germany
Description
- high-accuracy, high-resolution TOF mass spectrometer
- integrated Trapped Ion Mobility Spectrometry (TIMS) for additional gas-phase separation
- PASEF (Parallel Accumulation–Serial Fragmentation) for efficient ion use and increased fragmentation rates
- DIA-PASEF for reproducible, quantitative proteomics
- high sensitivity and fast scan speeds for complex samples
- LC systems
- nanoElute® 2 (Bruker Daltonics): long gradients for deep proteome analysis
- EvoSep One (Evosep): rapid and robust gradient methods
- HDX system
Applications
- bottom-up proteomics
- analysis of complex protein mixtures (e.g., cell lysates, serum)
- quantitative proteomic studies
LCT time-of-flight
Manufacturer
Waters/Micromass
MS Vision (Modifikationen)
Location
CSSB Centre for Structural Systems Biology, Hamburg, Germany
Description
- designed for high-mass measurements
- wide accessible mass range
- TOF detection
Applications
- native MS
- protein mass determination
- purity and composition checks
LEAP HDX PAL
Manufacturer
Axel Semrau by TRAJAN
Location
CSSB Centre for Structural Systems Biology, Hamburg
Description
- automated Hydrogen–Deuterium Exchange (HDX) workstation
- coupled to the timsTOF Pro
- LC system: Infinity 1260 HPLC system (Agilent)
- precise, temperature-controlled sample handling under HDX conditions
- enables time-resolved measurements of deuterium uptake
Applications
- analysis of protein dynamics and flexibility
- investigation of protein–ligand and protein–protein interactions
- localization of site-specific structural changes within proteins
Orbitrap Exploris™ 480
Manufacturer
Thermo Fisher Scientific
Location
Leibniz Institute of Virology, Hamburg, Germany
shared with Pietro Scaturro's research group
Description
- high-field asymmetric-waveform ion mobility spectrometry (FAIMS) for additional gas-phase separation
- high resolution enabled by Orbitrap™ technology
- LC systems
- Vanquis Neo UHPLC (Thermo Fisher Scientific): long gradients for deep proteome analysis
- EvoSep One (Evosep): rapid and robust gradient methods
Applications
- bottom-up proteomics
- phosphoproteomics
